Stability and State of Aggregation of Aqueous Fibrinogen and Dipalmitoylphosphatidylcholine Lipid Vesicles

二棕榈酰磷脂酰胆碱 动态光散射 化学 小泡 水溶液 浊度法 色散(光学) 色谱法 水动力半径 分析化学(期刊) 降水 化学工程 胶束 磷脂酰胆碱 纳米颗粒 有机化学 磷脂 生物化学 物理 光学 气象学 工程类
作者
Sook Heun Kim,Lilac Haimovich-Caspi,Liora Omer,Chi-Ming Yu,Yeshayahu Talmon,Nien‐Hwa Linda Wang,Elias I. Franses
出处
期刊:Langmuir [American Chemical Society]
卷期号:23 (10): 5657-5664 被引量:13
标识
DOI:10.1021/la0634701
摘要

The stability and state of aggregation of aqueous fibrinogen (FB) and dipalmitoylphosphatidylcholine (DPPC) vesicles in water or buffer at 25 °C were studied with dynamic light scattering (DLS), UV−vis spectroturbidimetry (ST), and cryo-transmission electron microscopy (cryo-TEM). In water, when 1000 ppm (0.10 wt %) DPPC dispersions were prepared with a protocol including extensive sonication, they contained mostly vesicles and were quite clear, transparent, and stable for at least 30 days. FB mixtures with water (0.075 wt %) were quite unstable and biphasic. They formed large aggregates which eventually precipitated. The addition of DPPC vesicles into these unstable FB dispersions reversed FB aggregation and precipitation and produced stable translucent microdispersions. The inferred lipid/protein aggregates were limited in size, with average diameters ranging from 200 to 300 nm. In buffer, DPPC dispersions were also clear and quite stable, with average dispersed particles diameter of ca. 90 nm. FB dissolved in aqueous buffer and formed transparent and stable solutions. Adding salt to an aggregated FB dispersion in water reversed the aggregation. FB aggregated and redissolved in the presence of the citrate and after the citrate was removed. There was no effect of citrate (present in FB initially) in the FB aggregation or redissolution. FB molecules in buffer form dimers or higher aggregates. Their average aggregation number is 2, determined with Rayleigh scattering analysis of turbidity data. The average hydrodynamic diameter of FB solutions from DLS was 30 nm. Mixing a stable FB solution in buffer and a stable DPPC dispersion in buffer produced highly unstable mixtures, in which large aggregates precipitated. These results have implications in understanding the interactions of lipids and proteins in many biological applications and food processing applications.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
FashionBoy应助风趣的灵枫采纳,获得10
1秒前
2秒前
yihuiqing发布了新的文献求助10
3秒前
likke发布了新的文献求助10
3秒前
嗝嗝发布了新的文献求助10
3秒前
小兔狸花昕完成签到,获得积分20
4秒前
IRer79完成签到,获得积分10
6秒前
彭于晏应助noah采纳,获得10
6秒前
青年才俊发布了新的文献求助10
6秒前
zhu发布了新的文献求助10
7秒前
波波蛋完成签到,获得积分10
7秒前
8秒前
9秒前
10秒前
maguodrgon发布了新的文献求助10
13秒前
科研通AI5应助shell采纳,获得80
13秒前
波波蛋发布了新的文献求助10
13秒前
14秒前
九鹤完成签到,获得积分10
14秒前
jsy完成签到,获得积分10
14秒前
量子星尘发布了新的文献求助10
15秒前
旷野发布了新的文献求助10
15秒前
15秒前
Owen应助浮云521采纳,获得10
15秒前
浮游应助muming采纳,获得10
15秒前
酷炫的凤妖完成签到 ,获得积分10
16秒前
17秒前
17秒前
19秒前
19秒前
19秒前
20秒前
21秒前
浮游应助勤劳的莆采纳,获得10
21秒前
22秒前
霜降发布了新的文献求助10
23秒前
jsy发布了新的文献求助10
23秒前
daring完成签到,获得积分10
23秒前
23秒前
上官若男应助maguodrgon采纳,获得10
25秒前
高分求助中
(应助此贴封号)【重要!!请各用户(尤其是新用户)详细阅读】【科研通的精品贴汇总】 10000
Zeolites: From Fundamentals to Emerging Applications 1500
Architectural Corrosion and Critical Infrastructure 1000
Early Devonian echinoderms from Victoria (Rhombifera, Blastoidea and Ophiocistioidea) 1000
By R. Scott Kretchmar - Practical Philosophy of Sport and Physical Activity - 2nd (second) Edition: 2nd (second) Edition 666
Energy-Size Reduction Relationships In Comminution 500
Principles Of Comminution, I-Size Distribution And Surface Calculations 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 内科学 生物化学 物理 计算机科学 纳米技术 遗传学 基因 复合材料 化学工程 物理化学 病理 催化作用 免疫学 量子力学
热门帖子
关注 科研通微信公众号,转发送积分 4941339
求助须知:如何正确求助?哪些是违规求助? 4207390
关于积分的说明 13077624
捐赠科研通 3986257
什么是DOI,文献DOI怎么找? 2182529
邀请新用户注册赠送积分活动 1198125
关于科研通互助平台的介绍 1110387