The purification of cardiac myofibrils with Triton X-100

Cardiac myofibrils treated with 1% Triton X-100 are virtually free of contamination by mitochondrial, sarcolemmal and sarcoplasmic reticular membranes. The ATPase activity of such myofibrils corresponds closely to that reported for cardiac actomyosin and the sensitivity of this ATPase activity to calcium control is normal.