兰克尔
成骨细胞
骨保护素
碱性磷酸酶
化学
骨钙素
运行x2
牙髓干细胞
破骨细胞
细胞生物学
间充质干细胞
内科学
分子生物学
内分泌学
生物
生物化学
受体
医学
体外
激活剂(遗传学)
酶
作者
Kazuyuki Yusa,Osamu Yamamoto,Mitsuyoshi Iino,Hiroshi Takano,Masayuki Fukuda,Zhiwei Qiao,Toshihiro Sugiyama
标识
DOI:10.1016/j.archoralbio.2016.07.010
摘要
Zinc is an essential element for proliferation, differentiation and survival in various cell types. In a previous study, we found that zinc ions released from zinc-modified titanium surfaces (eluted zinc ions; EZ) stimulate cell viability, osteoblast marker gene expression and calcium deposition in human bone marrow-derived mesenchymal cells (hBMCs). The aim of the present study was to investigate the effects of EZ on osteoblast differentiation among dental pulp stem cells (DPSCs) in vitro.In this study, we evaluated the effects of EZ on osteogenesis in DPSCs. Osteoblast and osteoclast marker gene expression was evaluated by real-time PCR. We also evaluated alkaline phosphatase (ALP) staining and calcium deposition.We found that EZ stimulated osteoblast marker gene (type I collagen, alkaline phosphatase (ALP), osteocalcin (OCN) and Runx2) expression, vascular endothelial growth factor A (VEGF-A), and TGF-beta signaling pathway-related gene expression after 7days of incubation. Osteoclastogenesis occurs in a receptor for activated nuclear-factor kappa B ligand (RANKL)/osteoprotegerin (OPG)-independent manner. Real-time PCR analysis revealed that EZ did not affect RANKL or OPG mRNA expression. It was also revealed that EZ induced alkaline phosphatase (ALP) staining and calcium deposition in DPSCs. Collectively, these results demonstrate the potential for clinical application to prospective treatment of bone diseases.
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