Identification of ITPR1 as a Hub Gene of Group 3 Medulloblastoma and Coregulated Genes with Potential Prognostic Values

小桶 基因 生物 计算生物学 髓母细胞瘤 生物信息学 基因表达 遗传学 转录组
作者
Pablo Ferreira das Chagas,Graziella Ribeiro de Sousa,Luciana Chain Veronez,Andrea Martins-da-Silva,Carolina Alves Pereira Corrêa,Gustavo Alencastro Veiga Cruzeiro,Luís Fernando Peinado Nagano,Rosane Gomes de Paula Queiróz,Suely Kazue Nagahashi Marie,Sílvia Regina Brandalise,Carlos Alberto Scrideli,Luíz Gonzaga Tone,Elvis Terci Valera
出处
期刊:Journal of Molecular Neuroscience [Springer Science+Business Media]
卷期号:72 (3): 633-641 被引量:7
标识
DOI:10.1007/s12031-021-01942-3
摘要

The Group 3 Medulloblastoma (Grp3-MB) is an aggressive molecular subtype with a high incidence of metastasis and deaths. In this study, were used an RNA sequencing data (RNA-Seq) from a Brazilian cohort of MBs to identify hub genes associated with the metastatic risk. Data validation were performed by using multiple large datasets from MBs (GSE85217, GSE37418, and EGAS00001001953). DESeq2 package in R software was used to identify the differentially expressed genes (DEGs) in our RNA-Seq data. The DEGs data were accessed to construct the modules/graphs of co-expression and to identify hub genes through Cytoscape platform. The coregulated genes were enriched by the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, and the protein-protein interaction (PPI) network was visualized by Cytoscape. The Kaplan-Meier plotter and ROC curves were used to validate the diagnostic and prognostic values of specific biomarkers identified through this model. We identified that inositol 1,4,5-trisphosphate receptor type 1 (ITPR1) as a downregulated hub gene, with a high diagnostic accuracy to Grp3-MBs and associated with tumor metastasis. In addition, we identified genes significantly correlated with ITPR1 that were associated with metastasis in Grp3-MB (ATP1A2, MTTL7A, and RGL1) and worst overall survival in MBs (ANTXR1 and RGL1). Our findings suggest that the ITPR1 hub gene is potentially involved in the metastatic process for Grp3-MB. Our data also provide evidence of targets that may serve as prognostic predictors and/or regulators for the metastatic process that maybe explored for further research of individualized therapy to Grp3-MBs.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
lslfreedom发布了新的文献求助10
1秒前
1秒前
Sun发布了新的文献求助10
1秒前
小马甲应助不靠谱采纳,获得10
1秒前
烟花应助GSGSG采纳,获得10
2秒前
lh完成签到,获得积分10
2秒前
GOW完成签到,获得积分10
2秒前
隔壁小曾发布了新的文献求助10
3秒前
3秒前
科研通AI2S应助山雀采纳,获得10
3秒前
huhu完成签到 ,获得积分10
4秒前
czj完成签到 ,获得积分10
4秒前
hz52发布了新的文献求助10
4秒前
九卫完成签到 ,获得积分20
5秒前
多喝热水完成签到,获得积分10
5秒前
月月月鸟伟完成签到,获得积分10
6秒前
情怀应助Naomi采纳,获得10
6秒前
6秒前
搬砖的化学男完成签到 ,获得积分0
6秒前
7秒前
SSSShawn发布了新的文献求助10
7秒前
威武的初兰完成签到 ,获得积分10
7秒前
义气的夏寒完成签到,获得积分10
8秒前
9秒前
9秒前
lixy完成签到,获得积分10
9秒前
乐乐乐乐乐乐应助斯文伊采纳,获得10
10秒前
小欢完成签到,获得积分10
10秒前
Ygy发布了新的文献求助10
10秒前
fdwonder完成签到,获得积分10
11秒前
11秒前
可可西里完成签到,获得积分10
11秒前
hz52完成签到,获得积分10
11秒前
推土机爱学习完成签到 ,获得积分10
11秒前
易止完成签到 ,获得积分10
11秒前
Iris完成签到 ,获得积分10
11秒前
Zhaowx完成签到,获得积分10
11秒前
12秒前
GSGSG发布了新的文献求助10
12秒前
ajun完成签到,获得积分10
12秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
A new approach to the extrapolation of accelerated life test data 1000
徐淮辽南地区新元古代叠层石及生物地层 500
Coking simulation aids on-stream time 450
北师大毕业论文 基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 390
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
Robot-supported joining of reinforcement textiles with one-sided sewing heads 360
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4015939
求助须知:如何正确求助?哪些是违规求助? 3555887
关于积分的说明 11319237
捐赠科研通 3288997
什么是DOI,文献DOI怎么找? 1812357
邀请新用户注册赠送积分活动 887882
科研通“疑难数据库(出版商)”最低求助积分说明 812044