Construction of Hohenbuehelia serotina polysaccharides-mucin nanoparticles and their sustain-release characteristics under simulated gastrointestinal digestion in vitro

In this study, Hohenbuehelia serotina polysaccharides-mucin nanoparticles (HSP-MC NPs) were fabricated based on hydrogen bonding and hydrophobicity effects for improving the bioavailability of HSP. The structural characteristics and morphology of HSP-MC NPs prepared by different conditions were respectively identified and observed. The results showed that HSP-MC NPs (HSP/MC, 1/1, w/w) presented the optimal physicochemical characteristics, with the encapsulation efficiency of 88.09 ± 0.01%, average particle size of 509.4 ± 9.76 nm and zeta potential of -20.6 ± 0.7 mV. Furthermore, HSP-MC NPs (HSP/MC, 1/1, w/w), belonged to non-crystalline substances, exhibited the excellent physicochemical stabilities against temperature, pH and ionic strength, and had the uniform spherical morphological characteristics. In addition, under simulated gastrointestinal digestion in vitro, HSP-MC NPs (HSP/MC, 1/1, w/w) showed the good sustained release performances, that might effectively improve the absorption rate of HSP. The present research is meaningful for designing the polysaccharides-loaded nano-delivery system based on natural non-toxic carrier that can be used in function food field.