微管
细胞生物学
微管聚合
生物物理学
体外
化学
微管蛋白
生物
生物化学
作者
Sylvie Gory‐Fauré,Julie Delaroche,Camille Cuveillier,Christian Delphin,Isabelle Arnal
出处
期刊:Methods in molecular biology
日期:2022-01-01
卷期号:: 375-383
标识
DOI:10.1007/978-1-0716-1983-4_24
摘要
Neuronal microtubules have long been known to contain intraluminal particles, called MIPs (microtubule inner proteins), most likely involved in the extreme stability of microtubules in neurons. This chapter describes a cryo-electron microscopy-based assay to visualize microtubules containing neuronal MIPs. We present two protocols to prepare MIPs-containing microtubules, using either in vitro microtubule polymerization assays or extraction of microtubules from mouse hippocampal neurons in culture.
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