Synthesis and Evaluation of Some Phenyl Substituted Azetidine Containing 1, 2, 4‐triazole Derivatives as Antibacterial Agents

A novel series of phenyl substituted azetidine containing 1, 2, 4‐triazole derivatives 7 ( a – j ) were synthesized and characterized by IR, 1HNMR, 13CNMR, and mass spectroscopy. Synthesized 1, 2, 4‐triazole derivatives were subsequently assayed in vitro to investigate their antibacterial activity against Staphylococcus aureus , Pseudomonas aeruginosa , and Escherichia coli using broth dilution method. Compounds 7c , 7d , and 7e exhibited potent inhibitory activities as compared to standard cefotaxime. Further, fluorescence spectral studies were also carried out to ascertain the antibacterial potential of compound 7c against two bacterial strains, that is, P. aeruginosa and S. aureus . In docking studies, all the compounds exhibited good docking scores between −12.04 and −11.36 kcal/mol and indicated that compounds could act through inhibition of bacterial DNA gyrase (PDB ID 3U2D). Among all, 7c has shown the maximum docking score and found in agreement to in vitro studies. In conclusion, synthesized 1, 2, 4‐triazole derivatives holds substantial caliber to be categorized as antibacterial agents.