生物
金黄色葡萄球菌
rpoB公司
转录组
微生物学
背景(考古学)
免疫系统
利福平
基因
表型
营养不良
溶解循环
基因组
遗传学
抗生素
细菌
病毒
基因表达
大肠杆菌
古生物学
16S核糖体RNA
作者
Guilherme Loss,Patrícia Martins-Simões,Florent Valour,Marina Farrel Côrtes,Luiz Gonzaga Paula de Almeida,Marine Bergot,Sophie Trouillet‐Assant,Jérôme Josse,Alan Diot,Emiliano P. Ricci,Ana Tereza Ribeiro de Vasconcelos,Frédéric Laurent
标识
DOI:10.3389/fcimb.2019.00363
摘要
Small colony variants (SCV) of Staphylococcus aureus have been reported as implicated in chronic infections. Here, we investigated the genomic and transcriptomic changes involved in the evolution from a wild-type to a SCV from in a patient with prosthetic joint infection relapse. The SCV presented a stable phenotype with no classical auxotrophy and the emergence of rifampicin resistance. Whole Genome Sequencing (WGS) analysis showed only the loss of a 42.5 kb phage and 3 deletions, among which one targeting the rpoB gene, known to be the target of rifampicin and to be associated to SCV formation in the context of a constitutively active stringent response. Transcriptomic analysis highlighted a specific signature in the SCV strain including a complex, multi-level strategy of survival and adaptation to chronicity within the host including a protection from the inflammatory response, an evasion of the immune response, a constitutively activated stringent response and a scavenging of iron sources.
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