库普弗电池
肝细胞
离心
细胞生物学
分离(微生物学)
肝细胞学
差速离心
肝细胞
细胞
生物
化学
分子生物学
生物化学
内科学
肝脏代谢
免疫学
体外
医学
微生物学
作者
Carolina Oses,Myriam Aouadi
出处
期刊:Methods in molecular biology
日期:2020-01-01
卷期号:: 1-10
被引量:3
标识
DOI:10.1007/978-1-0716-0704-6_1
摘要
Kupffer cells and hepatocytes maintain liver homeostasis. These cells could be separated based on their size and weight, by centrifugation using a density gradient after a liver perfusion. Here, we describe a methodology to isolate both Kupffer cells and hepatocytes from a single mouse, which provides the unique advantage of studying these two cell types from the same liver.
科研通智能强力驱动
Strongly Powered by AbleSci AI