ZNF667‐AS1, a positively regulating MEGF10, inhibits the progression of uveal melanoma by modulating cellular aggressiveness

Abstract Zinc finger protein 667‐antisense RNA 1 (ZNF667‐AS1) is a member of the C 2 H 2 zinc finger protein family. However, the exact effect of ZNF667‐AS1 in uveal melanoma (UM) progression has not been elucidated. The biological roles of ZNF667‐AS1 and MEGF10 were assessed using cell counting kit‐8 and flow cytometry. Quantitative reverse‐transcription polymerase chain reaction and Western blot analyses were conducted to measure the expression of subjects. ZNF667‐AS1 expression was significantly decreased in metastasized UM tissues and its low expression was related to poorer prognosis of UM patients. MEGF10 expression was positively associated with ZNF667‐AS1 expression. The inhibitory effect of ZNF667‐AS1 overexpression on UM cellular malignant behaviors could be reversed by MEGF10 knockdown, which was re‐ascertained by the detection of corresponding protein levels (p53, cyclin D1, Bcl‐2, and Bax). In conclusion, ZNF667‐AS1 might play an inhibitory role in the development of UM by regulating cellular aggressiveness, which was partially realized by positively regulating MEGF10.