Enhanced solubilization of solid organics and methane production by anaerobic digestion of swine manure under nano-bubble water addition

Nano-bubble water (NBW) refers to water with a large number of nanoscale particle bubbles. The aim of this work was to study the mechanism of NBW addition into the anaerobic digestion (AD) of swine manure (SM). The results showed that the cumulative methane production from the NBW added reactor was 192-225 mL/g-VS and 19-39% higher than the control group (without NBW addition). Based on the analysis of soluble organics, NBW addition not only accelerated hydrolysis rates of proteins and carbohydrates, but also enhanced the production of VFAs. Moreover, mechanism analysis reveals that NBW with higher spin-spin relaxation time and absolute value of zeta potential might promote enzyme activity and the hydrolysis of organic solids. Simultaneously, the electron transport system activity of the methanogenic communities and electric conductivity were enhanced by NBW addition. This work implies that NBW addition is promising for enhancing AD for enhancement of methane production.