TRPV1型
三氯乙酸
辣椒素
表皮(动物学)
瞬时受体电位通道
角质形成细胞
化学
医学
内分泌学
细胞生物学
生物化学
生物
受体
解剖
体外
作者
Hongjin Li,Nobuo Kanazawa,Ayako Kimura,Chikako Kaminaka,Nozomi Yonei,Yuki Yamamoto,Fukumi Furukawa
出处
期刊:European Journal of Dermatology
[John Libbey Eurotext]
日期:2012-09-01
卷期号:22 (5): 614-621
被引量:8
标识
DOI:10.1684/ejd.2012.1788
摘要
Transient receptor potential vanilloid 1 (TRPV1) is a highly polymodal TRP channel activated by various stimuli, including capsaicin, heat and acids. TRPV1 expression can be detected widely but is highest in sensory neurons and its activation alerts the body to noxious signals via neurogenic pain. Although TRPV1 is reportedly localized in the epidermis, it remains unclear how TRPV1 is involved in the chemical peeling processes with cytotoxic acids. Therefore, in this study, the role of TRPV1 on the effects of trichloroacetic acid (TCA) peeling was assessed using TRPV1-deficient mice. Following the confirmation of TRPV1 expression in murine keratinocytes with reverse transcription-polymerase chain reaction and immunohistochemistry, the effects of TCA on TRPV1-deficient mouse skin were compared with those on wild-type mouse skin. Our results indicated that TRPV1 expression was not required for TCA-induced DNA damage, as shown by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling, but was indispensable for the TCA-induced production of distinct growth factors and cytokines by keratinocytes. Ulceration after TCA peeling was actually more severe in the absence of TRPV1, suggesting that the TRPV1-mediated epidermal production of growth factors and cytokines affected the damaging and healing processes of TCA-peeled skin to induce rejuvenation.
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