Production of Multi-Purpose BAC Clones in the Novel Bacillus subtilis Based Host Systems

The BAC vector that replicates in Escherichia coli K-12 was first introduced in 1992 by Shizuya et al. More detailed information on current BAC applications may be referred to other chapters of this book. Because BACs can accommodate much longer DNA stretches than the plasmids for E. coli available at that time, as large as >100 kbp, they were suitable for the preparation of DNA libraries covering entire cellular genomes regardless of their original size (Fig. 1a). The initial goal for BACs was to provide materials for whole genome-sequencing projects and