乳糖
动物双歧杆菌
乳糖不耐受
双歧杆菌
真细菌
长双歧杆菌
食品科学
生物
益生元
肠道菌群
放线菌科
粪便
消化(炼金术)
微生物学
短双歧杆菌
细菌
乳酸菌
发酵
生物化学
化学
遗传学
色谱法
作者
Tao He,Marion G. Priebe,Yan Zhong,Chengyu Huang,Hermie J. M. Harmsen,Gerwin C. Raangs,Jean‐Michel Antoine,Gjalt W. Welling,Roel J. Vonk
标识
DOI:10.1111/j.1365-2672.2007.03579.x
摘要
Aims: Colonic metabolism of lactose may play a role in lactose intolerance. We investigated whether a 2‐week supplementation of Bifidobacterium longum (in capsules) and a yogurt enriched with Bifidobacterium animalis could modify the composition and metabolic activities of the colonic microbiota in 11 Chinese lactose‐intolerant subjects. Methods and Results: The numbers of total cells, total bacteria and the Eubacterium rectale/Clostridium coccoides group in faeces as measured with fluorescent in situ hybridization and the faecal β‐galactosidase activity increased significantly during supplementation. The number of Bifidobacterium showed a tendency to increase during and after supplementation. With PCR‐denaturing gradient gel electrophoresis, in subjects in which B. animalis and B. longum were not detected before supplementation, both strains were present in faeces during supplementation, but disappeared after supplementation. The degree of lactose digestion in the small intestine and the oro‐caecal transit time were not different before and after supplementation, whereas symptom scores after lactose challenge decreased after supplementation. Conclusions: The results suggest that supplementation modifies the amount and metabolic activities of the colonic microbiota and alleviates symptoms in lactose‐intolerant subjects. The changes in the colonic microbiota might be among the factors modified by the supplementation which lead to the alleviation of lactose intolerance. Significance and Impact of the Study: This study provides evidence for the possibility of managing lactose intolerance with dietary lactose (yogurt) and probiotics via modulating the colonic microbiota.
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