信使核糖核酸
融合蛋白
细胞生物学
绿色荧光蛋白
大肠杆菌
生物
基因
基因表达
核糖核酸
蛋白质生物合成
分子
黄色荧光蛋白
蛋白质亚细胞定位预测
生物物理学
化学
分子生物学
生物化学
重组DNA
有机化学
作者
Ji Yu,Jie Xiao,Xiaojia Ren,Kaiqin Lao,X. Sunney Xie
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:2006-03-16
卷期号:311 (5767): 1600-1603
被引量:930
标识
DOI:10.1126/science.1119623
摘要
We directly observed real-time production of single protein molecules in individual Escherichia coli cells. A fusion protein of a fast-maturing yellow fluorescent protein (YFP) and a membrane-targeting peptide was expressed under a repressed condition. The membrane-localized YFP can be detected with single-molecule sensitivity. We found that the protein molecules are produced in bursts, with each burst originating from a stochastically transcribed single messenger RNA molecule, and that protein copy numbers in the bursts follow a geometric distribution. The quantitative study of low-level gene expression demonstrates the potential of single-molecule experiments in elucidating the workings of fundamental biological processes in living cells.
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