生物
RNA干扰
转基因水稻
水稻
渗透性休克
脯氨酸
基因
转基因
转基因作物
细胞生物学
丙二醛
基因表达
生物化学
核糖核酸
氧化应激
氨基酸
作者
Ya-Guang Ke,Zhijun Yang,Shunwu Yu,Tianfei Li,Jinhong Wu,Huan Gao,Yaping Fu,Lijun Luo
标识
DOI:10.1007/s12374-013-0480-0
摘要
We identified a new dehydration-responsive element-binding protein gene, OsDREB6, which is an A-6 subgroup member of the DREB subfamily, of AP2/EREBP proteins, in Oryza sativa ssp. Japonica cv. Nipponbare. Expression pattern analysis indicated that OsDREB6 was induced in response to dehydration, high salinity, and most importantly by cold stress (4°C). Analysis using the yeast one-hybrid system showed that OsDREB6 had transcriptional activation activity and bound specifically to the DRE ciselement. Overexpression of OsDREB6 increased tolerance to osmotic, high salt, and cold stresses in transgenic rice whereas, OsDREB6 double-stranded RNA interference (RNAi) lines were found to be more sensitive to these stresses than wild-type rice. Additionally, sugar, proline and catalase levels in OsDREB6 RNAi lines were significantly lower than in the corresponding OsDREB6 overexpression lines and control lines after PEG treatment. In contrast, malondialdehyde (MDA) levels were significantly higher in RNAi lines than in control and overexpression lines. Furthermore, expression levels of some stress-related genes were altered in the transgenic plants. The data suggests that OsDREB6 may participate in the stress response by mediating the expression of specific genes, and may also be involved plant metabolism. Together, these results indicated that OsDREB6 plays an important role in the response of plants to a wide range of osmotic stresses.
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