组织工程
细胞生物学
间充质干细胞
心肌细胞
骨骼肌
体外
细胞培养
肌球蛋白
基质凝胶
C2C12型
内皮干细胞
生物
生物医学工程
解剖
生物化学
肌发生
医学
遗传学
作者
John S.K. Yuen,Brigid M. Barrick,Hailey DiCindio,Jaymie A. Pietropinto,David L. Kaplan
出处
期刊:ACS Biomaterials Science & Engineering
[American Chemical Society]
日期:2023-06-16
卷期号:9 (8): 4558-4566
标识
DOI:10.1021/acsbiomaterials.3c00358
摘要
A major challenge of engineering larger macroscale tissues in vitro is the limited diffusion of nutrients and oxygen to the interior. For skeletal muscle, this limitation results in millimeter scale outcomes to avoid necrosis. One method to address this constraint may be to vascularize in vitro-grown muscle tissue, to support nutrient (culture media) flow into the interior of the structure. In this exploratory study, we examine culture conditions that enable myogenic development and endothelial cell survival within tissue engineered 3D muscles. Myoblasts (C2C12s), endothelial cells (HUVECs), and endothelial support cells (C3H 10T1/2s) were seeded into Matrigel-fibrin hydrogels and cast into 3D printed frames to form 3D in vitro skeletal muscle tissues. Our preliminary results suggest that the simultaneous optimization of culture media formulation and cell concentrations is necessary for 3D cultured muscles to exhibit robust myosin heavy chain expression and GFP expression from GFP-transfected endothelial cells. The ability to form differentiated 3D muscles containing endothelial cells is a key step toward achieving vascularized 3D muscle tissues, which have potential use as tissue for implantation in a medical setting, as well as for future foods such as cultivated meats.
科研通智能强力驱动
Strongly Powered by AbleSci AI