噬菌体
噬菌体展示
质粒
噬菌体
生物
DNA
肽库
计算生物学
抗体
分子生物学
遗传学
基因
肽序列
大肠杆菌
出处
期刊:CSH Protocols
[Cold Spring Harbor Laboratory]
日期:2023-07-17
卷期号:2024 (8): pdb.over107756-pdb.over107756
被引量:3
标识
DOI:10.1101/pdb.over107756
摘要
A phagemid is a plasmid that contains the origin of replication and packaging signal of a filamentous phage. Following bacterial transformation, a phagemid can be replicated and amplified as a plasmid, using a double-stranded DNA origin of replication, or it can be replicated as single-stranded DNA for packaging into filamentous phage particles. The use of phagemids enables phage display of large proteins, such as antibody fragments. Phagemid pComb3 was among the first phage display vectors used for the generation and selection of antibody libraries in the 50-kDa Fab format, a monovalent proxy of natural antibodies. Affording a robust and versatile tool for more than three decades, phage display vectors of the pComb3 phagemid family have been widely used for the discovery, affinity maturation, and humanization of antibodies in Fab, scFv, and single-domain formats from naive, immune, and synthetic antibody repertoires. In addition, they have been used for broadening phage display to the mining of nonimmunoglobulin repertoires. This review examines conceptual, functional, and molecular features of the first-generation phage display vector pComb3 and its successors, pComb3H, pComb3X, and pC3C.
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