PI3K/AKT/mTOR通路
巨噬细胞
蛋白激酶B
细胞生物学
功能(生物学)
信号转导
化学
材料科学
生物
生物化学
体外
作者
Huanhuan Qiu,Huacui Xiong,Jiafu Zheng,Yuqi Peng,Chunhui Wang,Qing Hu,Fujian Zhao,Kexin Chen
出处
期刊:ACS Biomaterials Science & Engineering
[American Chemical Society]
日期:2024-05-20
卷期号:10 (6): 3923-3934
标识
DOI:10.1021/acsbiomaterials.4c00228
摘要
The repair of critical-sized bone defects continues to pose a challenge in clinics. Strontium (Sr), recognized for its function in bone metabolism regulation, has shown potential in bone repair. However, the underlying mechanism through which Sr2+ guided favorable osteogenesis by modulating macrophages remains unclear, limiting their application in the design of bone biomaterials. Herein, Sr-incorporated bioactive glass (SrBG) was synthesized for further investigation. The release of Sr ions enhanced the immunomodulatory properties and osteogenic potential by modulating the polarization of macrophages toward the M2 phenotype. In vivo, a 3D-printed SrBG scaffold was fabricated and showed consistently improved bone regeneration by creating a prohealing immunological microenvironment. RNA sequencing was performed to explore the underlying mechanisms. It was found that Sr ions might enhance the mitochondrial function of macrophage by activating PI3K/AKT/mTOR signaling, thereby favoring osteogenesis. Our findings demonstrate the relationship between the immunomodulatory role of Sr ions and the mitochondrial function of macrophages. By focusing on the mitochondrial function of macrophages, Sr2+-mediated immunomodulation sheds light on the future design of biomaterials for tissue regenerative engineering.
科研通智能强力驱动
Strongly Powered by AbleSci AI