可选择标记
生物
标记基因
重组酶
Cre重组酶
酵母
基因
遗传学
酿酒酵母
计算生物学
质粒
重组
转基因
转基因小鼠
出处
期刊:PubMed
日期:1994-06-01
卷期号:16 (6): 1086-8
被引量:40
摘要
A series of excisable marker cassettes has been constructed to facilitate recycling of selectable markers in yeast. These cassettes exploit the use of the Cre DNA recombinase to precisely excise the marker gene when desired. They are especially useful for making gene disruptions and then removing the marker gene to allow subsequent genetic manipulations with that same marker. Also described are a number of cre expression vectors that allow galactose-induced expression of the recombinase in yeast. The procedure is simple and allows rapid processing of large numbers of transformants.
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