Autophagy is required for toll-like receptor-mediated interleukin-8 production in intestinal epithelial cells

自噬 先天免疫系统 细胞生物学 生物 白细胞介素22 Toll样受体 受体 炎症 细胞因子 免疫系统 转染 白细胞介素 促炎细胞因子 白细胞介素8 细胞凋亡 免疫学 细胞培养 生物化学 遗传学
作者
Ishihara
出处
期刊:International Journal of Molecular Medicine [Spandidos Publications]
卷期号:27 (3) 被引量:24
标识
DOI:10.3892/ijmm.2011.596
摘要

Autophagy is an evolutionarily conserved process that maintains cellular homeostasis via synthesis, degradation, and subsequent recycling of cellular products under various physiological conditions. However, the link between autophagy and the innate immune system remains unknown. In the present study, we evaluated Toll-like receptor (TLR)-mediated autophagy induction in intestinal epithelial cells (IECs) and its relationship to interleukin (IL)-8 production. IEC-6, HCT-15, RAW264.7, and THP-1 cells were cultured with or without various TLR ligands, followed by evaluation of the expressions of pro-inflammatory cytokines [IL-8, cytokine-induced neutrophil chemoattractants (CINC)-2β, macrophage inflammatory protein (MIP)-2] by real-time PCR and ELISA. To reveal the status of autophagy in IECs and macrophages, light chain 3 (LC3)-II expression was examined using Western blotting and immunofluorescence with confocal microscopy. Also, to evaluate the influence of TLR ligands on autophagy-mediated innate-immune responses, autophagy-related gene (Atg)7 specific siRNA was transfected into intestinal epithelial cells and IL-8 expression was determined following exposure to various TLR ligands. Cells treated with the TLR ligands produced considerable amounts of pro-inflammatory cytokines (IL-8, CINC-2β, MIP-2). Furthermore, the basal levels of LC3-II were markedly higher in IECs as compared to those in macrophages. Our findings indicated that autophagy induction following TLR ligand stimulation was not significantly evident in IECs as compared to macrophages. In addition, Atg7 gene expression silencingled to down-regulation of TLR-mediated IL-8 expression in IECs, which indicates a potential role of autophagy in generating innate-immune responses. In conclusion, autophagy may be an important intracellular machinery for inducing the innate immune system in IECs.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
尘羽临风完成签到,获得积分10
1秒前
随影相伴完成签到 ,获得积分10
2秒前
2秒前
勇敢的前进完成签到,获得积分10
2秒前
飞快的寻云完成签到,获得积分10
2秒前
小孙发布了新的文献求助10
3秒前
amino完成签到,获得积分10
3秒前
3秒前
婷123发布了新的文献求助10
4秒前
梦与叶落完成签到 ,获得积分10
4秒前
5秒前
5秒前
汉堡包应助马登采纳,获得30
6秒前
6秒前
yyymmma应助兴奋的故事采纳,获得10
7秒前
7秒前
7秒前
枫威发布了新的文献求助10
8秒前
欢城完成签到,获得积分10
9秒前
顾矜应助azheng采纳,获得10
9秒前
star完成签到 ,获得积分10
9秒前
Karma完成签到,获得积分10
9秒前
9秒前
星星发布了新的文献求助10
10秒前
10秒前
10秒前
11秒前
一只会打呼的喵完成签到,获得积分20
12秒前
12秒前
顾矜应助房房房破防啦采纳,获得10
13秒前
14秒前
15秒前
kk发布了新的文献求助10
15秒前
asdfghjkl完成签到,获得积分10
16秒前
17秒前
锅子碗XXX完成签到,获得积分10
17秒前
ymy123发布了新的文献求助10
18秒前
18秒前
19秒前
20秒前
高分求助中
The late Devonian Standard Conodont Zonation 2000
Nickel superalloy market size, share, growth, trends, and forecast 2023-2030 2000
The Lali Section: An Excellent Reference Section for Upper - Devonian in South China 1500
Very-high-order BVD Schemes Using β-variable THINC Method 870
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 800
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 800
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3256273
求助须知:如何正确求助?哪些是违规求助? 2898535
关于积分的说明 8301409
捐赠科研通 2567721
什么是DOI,文献DOI怎么找? 1394646
科研通“疑难数据库(出版商)”最低求助积分说明 652913
邀请新用户注册赠送积分活动 630557