材料科学
乙二醇
组织工程
明胶
生物医学工程
甲基丙烯酸酯
PEG比率
纳米技术
聚合物
化学工程
聚合
复合材料
化学
生物化学
医学
工程类
财务
经济
作者
Peter Kim,Alex E. Yuan,Ki‐Hwan Nam,Alex Jiao,Deok‐Ho Kim
出处
期刊:Biofabrication
[IOP Publishing]
日期:2014-04-10
卷期号:6 (2): 024112-024112
被引量:77
标识
DOI:10.1088/1758-5082/6/2/024112
摘要
Although synthetic polymers are desirable in tissue engineering applications for the reproducibility and tunability of their properties, synthetic small diameter vascular grafts lack the capability to endothelialize in vivo. Thus, synthetically fabricated biodegradable tissue scaffolds that reproduce important aspects of the extracellular environment are required to meet the urgent need for improved vascular grafting materials. In this study, we have successfully fabricated well-defined nanopatterned cell culture substrates made of a biodegradable composite hydrogel consisting of poly(ethylene glycol) dimethacrylate (PEGDMA) and gelatin methacrylate (GelMA) by using UV-assisted capillary force lithography. The elasticity and degradation rate of the composite PEG-GelMA nanostructures were tuned by varying the ratios of PEGDMA and GelMA. Human umbilical vein endothelial cells (HUVECs) cultured on nanopatterned PEG-GelMA substrates exhibited enhanced cell attachment compared with those cultured on unpatterned PEG-GelMA substrates. Additionally, HUVECs cultured on nanopatterned PEG-GelM substrates displayed well-aligned, elongated morphology similar to that of native vascular endothelial cells and demonstrated rapid and directionally persistent migration. The ability to alter both substrate stiffness and degradation rate and culture endothelial cells with increased elongation and alignment is a promising next step in recapitulating the properties of native human vascular tissue for tissue engineering applications.
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