蒲公英
角鲨烯
转录因子
生物
茉莉酸甲酯
萜烯
生物化学
转基因
蒲公英
基因
生物合成
细胞生物学
医学
病理
中医药
替代医学
作者
Tingyao Liu,Jingjing Liao,Min Shi,Li Li,Qun Liu,Xin Cui,Ning Wei,Guoyin Kai
出处
期刊:Plant Science
[Elsevier]
日期:2023-01-01
卷期号:326: 111506-111506
被引量:5
标识
DOI:10.1016/j.plantsci.2022.111506
摘要
Dandelion is a well-known traditional medical herb, also used as functional food. Dandelion possesses many medical properties, such as anti-bacterial and antioxidant activity and contains a variety of triterpenes, such as α-amyrin, β-amyrin, taraxerol and taraxasterol. In this study, we found that triterpenes biosynthesis was promoted by methyl jasmonate (MeJA), while the transcriptional mechanism underlying triterpenes biosynthesis was rarely investigated. Here, a MeJA-induced bHLH transcription factor TaMYC2 was identified. The content of taraxasterol and taraxerol in dandelion was obviously enhanced in overexpression TaMYC2 transgenic lines and expression level of the squalene synthase gene (TaSS) was elevated to about 3-5 folds compared with the control lines. Dual-LUC, Y1H and EMSA experiments revealed that TaMYC2 bound to the E-box motif in the promoter of TaSS and activated its transcription. Taken together, this study suggested that TaMYC2 acted as a positive regulator for bioengineering approaches to produce high content triterpenes-producing dandelions.
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