树枝状大分子
转染
小RNA
基因传递
阳离子聚合
化学
遗传增强
癌症
体内
体外
癌症研究
生物物理学
纳米技术
组合化学
细胞生物学
材料科学
生物化学
生物
基因
高分子化学
生物技术
遗传学
作者
Yu Zou,Siyan Shen,Andrii Karpus,Huxiao Sun,Régis Laurent,Anne‐Marie Caminade,Mingwu Shen,Serge Mignani,Xiangyang Shi,Jean‐Pierre Majoral
出处
期刊:Biomacromolecules
[American Chemical Society]
日期:2024-01-05
卷期号:25 (2): 1171-1179
被引量:2
标识
DOI:10.1021/acs.biomac.3c01169
摘要
The development of nonviral dendritic polymers with a simple molecular backbone and great gene delivery efficiency to effectively tackle cancer remains a great challenge. Phosphorus dendrimers or dendrons are promising vectors due to their structural uniformity, rigid molecular backbones, and tunable surface functionalities. Here, we report the development of a new low-generation unsymmetrical cationic phosphorus dendrimer bearing 5 pyrrolidinium groups and one amino group as a nonviral gene delivery vector. The created AB5-type dendrimers with simple molecular backbone can compress microRNA-30d (miR-30d) to form polyplexes with desired hydrodynamic sizes and surface potentials and can effectively transfect miR-30d to cancer cells to suppress the glycolysis-associated SLC2A1 and HK1 expression, thus significantly inhibiting the migration and invasion of a murine breast cancer cell line in vitro and the corresponding subcutaneous tumor mouse model in vivo. Such unsymmetrical low-generation phosphorus dendrimers may be extended to deliver other genetic materials to tackle other diseases.
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