荧光
猝灭(荧光)
化学
催化作用
氯霉素
DNA
组合化学
产品(数学)
光化学
天然产物
生物化学
物理
光学
几何学
数学
抗生素
作者
Yu Zou,Wanshan Wang,Feifei Duan,Wen Yun,Hong Chen,Yiping Li,Lizhu Yang
标识
DOI:10.1016/j.foodchem.2024.141144
摘要
An ultrasensitive fluorescence detection strategy of chloramphenicol (CAP) was developed based on product catalysis of tetrahedral DNA framework (TDF) and fluorescent quenching of MIL-101(Fe). The product was used to catalyze the reaction. As the concentration of catalyst increased, the reaction time was significantly shorted to 21 min which was much shorter than other isothermal amplification technologies. Moreover, the multiple fluorophores of TDF and high efficient quenching ability of MIL-101(Fe) provided better performance with a linear range for CAP detecting from 1.6 pM to 80 pM and the limit of detection (LOD) as low as 0.67 pM. In addition, it also demonstrated good specificity and resistance to interference from other related antibiotics. Importantly, this strategy exhibited satisfactory relative standard deviation and recovery results for practical application, exhibiting a favorable application prospect in CAP analysis.
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