密螺旋体
细菌外膜
抗体
微生物学
生物
免疫系统
抗血清
病毒学
免疫学
梅毒
生物化学
基因
大肠杆菌
人类免疫缺陷病毒(HIV)
作者
Melissa J. Caimano,Melissa J. Caimano,Isabel C. Orbe,Crystal F. Vicente,Carson J. La Vake,André Alex Grassmann,M. Anthony Moody,Justin D. Radolf,Kelly L. Hawley
出处
期刊:PLOS Pathogens
[Public Library of Science]
日期:2024-12-23
卷期号:20 (12): e1012443-e1012443
标识
DOI:10.1371/journal.ppat.1012443
摘要
The global resurgence of syphilis has created a potent stimulus for vaccine development. To identify potentially protective antibodies against Treponema pallidum ( TPA ), we used Pyrococcus furiosus thioredoxin ( Pf Trx) to display extracellular loops (ECLs) from three TPA outer membrane protein families (outer membrane factors for efflux pumps, eight-stranded β-barrels, and FadLs) to assess their reactivity with immune rabbit serum (IRS). We identified five immunodominant loops from the FadL orthologs TP0856, TP0858 and TP0865 by immunoblotting and ELISA. Rabbits and mice immunized with these five Pf Trx constructs produced loop-specific antibodies that promoted opsonophagocytosis of TPA by rabbit peritoneal and murine bone marrow-derived macrophages at levels comparable to IRS and mouse syphilitic serum. Heat-inactivated IRS and loop-specific rabbit and mouse antisera also impaired viability, motility, and cellular attachment of spirochetes during in vitro cultivation. The results support the use of ECL-based vaccines and suggest that loop-specific antibodies promote spirochete clearance via Fc receptor-independent as well as Fc receptor-dependent mechanisms.
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