Long‐term antiviral therapy is associated with changes in the profile of transcriptionally active HBV integration in the livers of patients with CHB

恩替卡韦 HBeAg 乙型肝炎表面抗原 医学 乙型肝炎病毒 抗病毒治疗 病毒学 cccDNA 乙型肝炎 阿德福韦 效价 聚乙二醇干扰素 免疫学 病毒 内科学 慢性肝炎 拉米夫定 利巴韦林
作者
Xiajie Wen,Xiaoning Wu,Yameng Sun,Jialing Zhou,Guiwen Guan,Shuyan Chen,Shan Shan,Hong Ma,Xinyan Zhao,Yu Wang,Xiaojuan Ou,Hong You,Ju‐Tao Guo,Fengmin Lu,Jidong Jia
出处
期刊:Journal of Medical Virology [Wiley]
卷期号:96 (6) 被引量:3
标识
DOI:10.1002/jmv.29606
摘要

Abstract Hepatitis B virus (HBV) integration exists throughout the clinical course of chronic hepatitis B (CHB). This study investigated the effects of long‐term antiviral therapy on the level and profiles of transcriptionally active HBV integration. Serial liver biopsies and paired blood samples were obtained from 16, 16, and 22 patients with CHB at baseline, 78, and 260 weeks of entecavir monotherapy or combined with pegylated interferon alfa, respectively. Serum HBV biomarkers were longitudinally assessed. RNA‐seq and HIVID2 program was used to identify HBV‐host chimeric RNAs transcribed from integrated DNA. The counts of HBV integration reads were positively related to both serum HBV DNA levels ( r = 0.695, p = 0.004) and HBeAg titers ( r = 0.724, p = 0.021) at baseline, but the positive correlation exited only to the serum HBsAg levels after 260 weeks of antiviral therapy ( r = 0.662, p = 0.001). After 78 weeks of antiviral therapy, the levels of HBV integration expression decreased by 12.25 folds from baseline. The viral junction points were enriched at the S and HBx genes after the long‐term antiviral therapy. HBs‐FN1 became one of the main transcripts, with the mean proportion of HBs‐FN1 in all integrated expression increased from 2.79% at baseline to 10.54% at Week 260 of antiviral treatment. Antiviral therapy may reduce but not eliminate the HBV integration events and integration expression. Certain integration events, such as HBs‐FN1 can persist in long‐term antiviral treatment.
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