Healing Effect and Underlying Mechanism of Silver Nanoparticles Prepared Using Carboxymethyl Chitosan under Solar Irradiation

In our previous study, silver nanoparticles were prepared using AgNO 3 and carboxymethyl chitosan (CMCS) which is commercially available with solar irradiation.In this study, the efficacy and safety of silver nanoparticles prepared by this method were evaluated for healing wounds in rats with diabetes.We also attempted to determine the underlying mechanism and influencing factors of the silver nanomaterial preparation method.Compared with growth factors, silver nanoparticles exhibit better healing effects for rats with diabetes.No remnant silver ions were detected in the major organs of these rats after the application of silver nanoparticles.Silver nanoparticles prepared using CMCS are less toxic than those prepared from the conventional method, promote the proliferation of skin fibroblasts, and are promising as a topical medication for infected wounds.An obvious breakage process of the particles is observed during the growth of nanocrystalline silver in CMCS.In this study, we also attempted to determine whether this method is suitable for synthesizing silver nanoparticles using N-trimethyl chitosan chloride and sodium alginate were used in this particular experiment.The characteristic UV-vis absorbance peak of silver nanoparticles was found only in the reaction mixture containing N-trimethyl chitosan chloride.Our study demonstrates that free radicals are the key factor in this silver nanoparticle preparation method.