活性氧
过氧化氢
葡萄糖氧化酶
激进的
核化学
化学
过氧化物酶
光热治疗
生物相容性
光动力疗法
纳米技术
材料科学
生物化学
酶
有机化学
作者
Girum Getachew,Chiranjeevi Korupalli,Akash S. Rasal,Jia‐Yaw Chang
标识
DOI:10.1016/j.compositesb.2021.109364
摘要
In this study, the reactive oxygen species (ROS) scavenging or generation ability of the carbon dots (CDs) was regulated by incorporating with heteroatoms (Cu and Cl ions). The pristine CDs were found to be powerful anti-oxidants to scavenge ROS, with half-maximal inhibitory concentrations (IC50) of •O2− and •OH radicals estimated to be 6.89 and 6.12 μg/mL, respectively, whereas Cu and Cl co-doped CDs (CuCl-CDs) possessed not only ROS generation ability upon laser irradiation for photodynamic therapy (PDT), but also peroxidase-mimic activity that generates oxidative •OH from hydrogen peroxide (H2O2) for chemodynamic therapy (CDT). Moreover, the colorimetric assay, 1O2 emission peak, and ESR results supported the efficient production of •O2−, •OH, and 1O2 radicals. Furthermore, CuCl-CDs with ROS-generating abilities and peroxidase-mimetic properties were successfully integrated with polydopamine (PDA) and glucose oxidase (GOx) to fabricate multifunctional GOx/CuCl-CD@PDA-PEG (GCP) nanocomposites with hydrodynamic sizes of 135.5 nm. These novel GCP nanocomposites possessed satisfactory photothermal conversion efficacies (η = 24.4%) and gave a high yield of ROS via the combination of H2O2 and laser irradiation. Moreover, the presence of GOx in GCP nanocomposites enables these compounds to decrease the intracellular glucose levels for starvation therapy and the enzymatic cascade activity for enhanced ROS-mediated therapy. In vitro studies and confirmed that these GCP nanocomposites displayed good biocompatibility with concentrations from 100 to 1000 ppm, but induced 90% reduction in B16F1 cell viability at 200 ppm via the cooperative effects of CDT, phototherapeutic effect, and starvation therapy.
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