Selection of sensitive seeds for evaluation of compost maturity with the seed germination index

The seed germination index (GI) is a widely used indicator of compost maturity and is a required index in many national standards. However, seeds of different species vary markedly in sensitivity to the biological toxicity of compost. Therefore, suitable seed selection is essential for evaluation of compost maturity with the GI. This study systematically investigated the germination percentage and root length for seeds of 17 species incubated in deionized water for 48 h at 25 °C in the dark. Based on the germination percentage, seeds of eight species (white radish, cucumber, fruit radish, edible rape, round radish, hybrid cucumber, cress, and Chinese cabbage) were selected for determination of the GI of chicken manure composted with differing proportions of tobacco powder and mushroom substrate. The GI of hybrid cucumber seeds showed a significant positive correlation with temperature and pH, and a significant negative correlation with E4/E6 ratio and ammonium-nitrogen content. The change in GI of hybrid cucumber seeds during composting and the GI value at the completion of composting were consistent with other maturity indicators. Among the tested seeds, the biological toxicity of the compost was best characterized by hybrid cucumber seeds, which thus represented a sensitive and reliable seed suitable for evaluation of compost maturity with the GI.