间充质干细胞
材料科学
组织工程
生物医学工程
心脏病
心脏病学
内科学
医学
病理
作者
Kai Kang,Jun-bo Chuai,Xie Baodong,Jianzhong Li,Hui Qü,Hua Wu,Shaohong Fang,Jinjin Cui,Duo Li,Jincheng Han,Tianhui Cao,Xiaoping Leng,Hai Tian,Ren‐Ke Li,Shulin Jiang
出处
期刊:Biomaterials
[Elsevier]
日期:2019-11-06
卷期号:230: 119574-119574
被引量:18
标识
DOI:10.1016/j.biomaterials.2019.119574
摘要
Engineered heart tissues (EHTs) are regarded as being the most promising alternative to synthetic materials, and autologous mesenchymal stem cells (MSCs) are widely used as seeding cells. However, few studies have evaluated the feasibility of using MSCs from patients with cyanotic congenital heart disease (C-CHD) as seeding cells for EHTs, in comparison with cells from patients of acyanotic congenital heart disease (A-CHD). In the present study, we cultured MSCs from A-CHD and C-CHD patients in normoxia or hypoxia conditions, and compared their pro-angiogenic, anti-apoptotic and inflammation-modulatory potentials. In vivo, we seeded the cells into collagen patches conjugated with, or without, proangiogenic cytokines, which were used to repair the right ventricular outflow tract (RVOT) of rats. The in vitro results showed that C-CHD MSCs expressed higher levels of VEGFA and VEGFR2, and secreted more pro-angiogenic and anti-inflammatory cytokines under hypoxic conditions. On the other hand, apoptosis-related genes from C-CHD MSCs were modulated adaptably, converting these cells into an anti-apoptotic phenotype. In vivo studies demonstrated that in 4 weeks after RVOT reconstruction, cytokine-immobilized patches seeded with C-CHD MSCs exhibited preserved morphology, prolonged cell survival and enhanced angiogenesis compared to A-CHD MSCs. C-CHD MSCs that undergo naturally hypoxic precondition present a better cell source for EHTs, which would provide a promising individualized biomaterial for C-CHD patients.
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