A novel strategy to collect expressed prostatic secretion

Objective To establish a simple, rapid and reliable method for collecting expressed prostatic secretion (EPS). Methods Thirty-five Beagle dogs were randomly divided into 7 groups (n=5 each). After construction of urinary tract infection animal model, urine, EPS and prostate tissue were collected for bacterial culture and microscopic examination of white blood cells. Besides, urethra and prostate tissue were obtained for histopathological and electron microscopic examination, and evaluation of urethral mucosa irritation. Results EPS results showed that bacterial test in the third group was positive, that in the other four groups was negative, Urine results in group 1 and 2 were negative, and those in the group 3-5 were positive. There was not significant (t=0.871, P=0.409) difference between the sixth groups [(3.60±0.55)/HP] and the seventh groups [(3.33±0.41)/HP]. The results above were confirmed by histopathological and electron microscopic examinations. The results also showed that JUS long-acting antibacterial material had a slight irritation to mucous membrane. Conclusion The EPS collected by JUC can exclude the possibility of being contaminated by urethra (false positive) and the interference of urethra (false negative), suggesting a time-wise feasible. Key words: Expressed prostatic secretion; JUC long-asting antibacterial material; Beagle urinary tract infection model; Prostatitis