Immunoprecipitation of DNA:RNA Hybrids Using the S9.6 Antibody

Formation of DNADNA:RNARNA hybrids or R-loopsR-loops contributes to numerous biologic processes. The development of the S9.6 antibody makes the analysis of R-LoopsR-loops (DNADNA:RNARNA hybrids) possible through immunoprecipitationImmunoprecipitation. Here, we describe the isolation of DNADNA:RNARNA hybrid structures using the S9.6 antibody. Using this protocol, both the DNA and RNA binding partners of the R-loop can be analyzed via qPCR, whole genome sequencing, or other methods.