Factors affecting the timing and imprinting of replication on a mammalian chromosome

生物 复制计时 印记(心理学) 遗传学 染色质 同源染色体 DNA复制 染色体 人口 基因 人口学 社会学
作者
Wendy A. Bickmore,Andrew D. Carothers
出处
期刊:Journal of Cell Science [The Company of Biologists]
卷期号:108 (8): 2801-2809 被引量:83
标识
DOI:10.1242/jcs.108.8.2801
摘要

Fluorescence in situ hybridisation has been used to follow replication of the short arm of human chromosome 11 using chromosome anomalies to distinguish the maternally-and paternally-derived homologues. The temporal difference in replication timing within and between chromosomes has been estimated by combining S phase detection with dual colour fluorescence in situ hybridisation. Proximal regions of 11p, including the WT1 gene, tend to replicate earlier on the maternally-derived chromosome than on the paternally-derived homologue. More distal parts of 11p (including the IGF2 gene) have the opposite imprint. The average difference in replication timing between homologous loci in the population of cells is small compared to the differences between loci along a single chromosome. The imprint is not strictly adhered to since many nuclei have hybridisation patterns opposite to the trend within the population. The nature of the imprinting signal has been investigated. Absolute replication time, but not the imprint, was affected by azacytidine, an inhibitor of DNA methylation. The replication imprint was modified by treatments that inhibit histone deacetylation. We suggest that replication imprinting reflects differences in chromatin structure between homologues.

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