Minor new withanolides, daturametelins C, D, E, F and G-Ac, have been isolated from the methanolic extract of the fresh aerial parts of Datura metel L. (Solanaceae) and their structures have been determined mainly by spectroscopic means. Daturametelins E anf F are the first withanolides having a 1-one-3β-O-sulfate structure in ring A.