等位基因排除
生物
同型
免疫球蛋白轻链
免疫球蛋白类转换
抗体
免疫球蛋白重链
基因
B细胞
基因重排
基因座(遗传学)
遗传学
分子生物学
重组激活基因
重组
T细胞
免疫系统
单克隆抗体
T细胞受体
作者
Pedro Perdiguero,Esther Morel,Patricia Díaz‐Rosales,Carolina Tafalla
出处
期刊:iScience
[Elsevier]
日期:2021-06-01
卷期号:24 (6): 102615-102615
被引量:8
标识
DOI:10.1016/j.isci.2021.102615
摘要
B cells express a unique antibody protein which comprises two pairs of immunoglobulin (Ig) heavy (H) and light (L) chains. In addition to an invariable constant (C) region, IgH and IgL chains encompass a variable (V) region mediating antigen binding. This unique region stems from Ig V(D)J gene recombination, which generates diversity by assembling these gene segments into VHDJH and VLJL genes. To ensure that one B cell only expresses one antibody, VHDJH rearrangement occurs only in one IgH locus (allelic exclusion), whereas VLJL rearrangement only in either the κ or λ locus (isotype exclusion). However, teleosts express multiple IgLs encoded by distinct CL genes. Using single-cell transcriptomics, we have demonstrated the transcription of distinct rearranged VLJLCL genes in single rainbow trout B cells. Our results highlight the laxity of isotype exclusion in teleosts and strongly suggest that fish B cells can produce antibodies of different specificities.
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