钙黄绿素
荧光
生物物理学
细胞培养
化学
细胞
细胞生物学
缝隙连接
生物
生物化学
细胞内
光学
遗传学
物理
作者
Gary S. Goldberg,John F. Bechberger,Christian C. Naus
出处
期刊:PubMed
日期:1995-03-01
卷期号:18 (3): 490-7
被引量:119
摘要
We describe a simple method for evaluating gap junctional communication (GJC) between cells in culture. The procedure involves pre-loading cells with two fluorescent dyes: calcein and DiI. Calcein is able to pass through gap junctions, while DiI is not. These pre-loaded cells are then plated with unlabeled cells. The number of cells receiving calcein from each pre-loaded cell can then be quantified after the cells settle on the plate. Potent and reversible inhibitors of GJC can be used in this system to evaluate dye transfer within a given period of time.
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