溶菌酶
抗菌剂
鲍曼不动杆菌
细菌细胞结构
纳米团簇
致病菌
抗生素耐药性
粪肠球菌
化学
抗生素
微生物学
细菌
抗药性
金黄色葡萄球菌
生物
生物化学
铜绿假单胞菌
遗传学
有机化学
作者
Wei-Yu Chen,Jia-Horng Lin,Sarah Samadi,Liyang Luo,Eric Wei-Guang Diau,Yu-Chie Chen
出处
期刊:Nanomedicine
日期:2010-07-01
卷期号:5 (5): 755-764
被引量:147
摘要
Our aim was to demonstrate that lysozyme-directed generation of gold nanoclusters (Au NCs) are potential antimicrobial agents for antibiotic-resistant bacteria and broad labeling agents for pathogenic bacteria.Lysozyme is an enzyme that is capable of hydrolyzing the cell walls of bacteria. In this study, we demonstrated the generation of functional Au NCs by using lysozyme as the sequester and the reducing agent for Au precursors at 40 degrees C. In addition, to shorten the reaction time, the reaction was conducted under microwave irradiation within a short period of time for the first time.The bioactivity of the lysozyme on the Au NCs was retained. Therefore, the as-prepared lysozyme-Au NCs with desirable fluorescence feature were successfully employed to be broad-band labeling agents for pathogenic bacteria. Furthermore, we also demonstrated that the lysozyme-Au NCs can be used to effectively inhibit the cell growth of notorious antibiotic-resistant bacteria, including pan-drug-resistant Acinetobacter baumannii and vancomycin-resistant Enterococcus faecalis.The potential of employing the lysozyme-Au NCs for bacterial labeling and as antimicrobial agents is expected.
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