荧光
化学
生物物理学
共价键
利用
纳米技术
生物系统
荧光标记
绿色荧光蛋白
荧光蛋白
生物化学
计算机科学
材料科学
生物
物理
有机化学
基因
量子力学
计算机安全
出处
期刊:Methods in molecular biology
日期:2011-01-01
卷期号:: 101-120
被引量:37
标识
DOI:10.1007/978-1-61779-282-3_6
摘要
Many single-molecule experimental techniques exploit fluorescence as a tool to investigate conformational dynamics and molecular interactions or track the movement of proteins in order to gain insight into their biological functions. A prerequisite to these experimental approaches is to graft one or more fluorophores on the protein of interest with the desired photophysical properties. Here, we present detailed procedures for the current most efficient methods used to covalently attach fluorophores to proteins. Alternative direct and indirect labeling strategies are also described.
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