The Screening of Sporogenous Medium and Haploid Separation of Saccharomyces Cerevisiae

To obtain a haploid genetics hybrid breeding of 1450(S.Cerevisiae),Spore-producing cultivation experiments of 1450 were done by using different culture media and under different conditions.The results showed that the optimum sporulation medium and cultivation time were McClary medium and 6 days,the cultivation temperature must be controlled at 25～28 ℃.Under the above conditions,the sporulation rate could reach to about 70 %.A yeast strain S-3 as a genetics hybrid breeding was isolated by comparasion of the fermentation capability and performance of haploid.