生物
矮化
赤霉素
突变体
氧化酶试验
生物合成
基因
拟南芥
生物化学
脱落酸
拟南芥
植物茎
植物
遗传学
酶
作者
Zuliang Li,Baozhu Li,Junli Zhang,Hongliang Wang,Mao Wang,Siyi Guo,Pengtao Wang,Zhi Li,David W. Galbraith,Dandan Li,Chun‐Peng Song
出处
期刊:Crop Journal
[Elsevier]
日期:2023-12-01
卷期号:11 (6): 1742-1751
被引量:1
标识
DOI:10.1016/j.cj.2023.04.008
摘要
Gibberellin (GA) functions in plant growth and development. However, genes involved in the biosynthesis and regulation of GA in crop plants are poorly understood. We isolated the mutant gad5-1 (GA-Associated Dwarf 5), characterized by dwarfing, short internodes, and dark green and short leaves. Map-based gene cloning and allelic verification confirmed that ZmGAD5 encodes ent-kaurenoic acid oxidase (KAO), which catalyzes KA (ent-kaurenoic acid) to GA12 conversion during GA biosynthesis in maize. ZmGAD5 is localized to the endoplasmic reticulum and is present in multiple maize organs. In gad5-1, the expression of ZmGAD5 is severely reduced, and the levels of the direct substrate of KAO, KA, is increased, leading to a reduction in GA content. The abnormal phenotype of gad5-1 was restored by exogenous application of GA3. The biomass, plant height, and levels of GA12 and GA3 in transgenic Arabidopsis overexpressing ZmGAD5 were increased in comparison with the corresponding controls Col-0. These findings deepen our understanding of genes involved in GA biosynthesis, and could lead to the development of maize lines with improved architecture and higher planting-density tolerance.
科研通智能强力驱动
Strongly Powered by AbleSci AI