创伤性脑损伤
免疫印迹
代谢物
代谢组学
病理
化学
多巴胺
药理学
医学
生物化学
内科学
色谱法
精神科
基因
作者
En Hu,Tao Tang,You‐mei Li,Teng Li,Lin Zhu,Ruoqi Ding,Yao Wu,Qing Huang,Wei Zhang,Qian Wu,Yang Wang
摘要
Abstract Introduction Spatial changes of amine metabolites and histopathology of the whole brain help to reveal the mechanism of traumatic brain injury (TBI) and treatment. Methods A newly developed liquid microjunction surface sampling–tandem mass tag–ultra performance liquid chromatography–mass spectrometry technique is applied to profile brain amine metabolites in five brain regions after impact‐induced TBI at the subacute stage. H&E, Nissl, and immunofluorescence staining are performed to spatially correlate microscopical changes to metabolic alterations. Then, bioinformatics, molecular docking, ELISA, western blot, and immunofluorescence are integrated to uncover the mechanism of Xuefu Zhuyu decoction (XFZYD) against TBI. Results Besides the hippocampus and cortex, the thalamus, caudate‐putamen, and fiber tracts also show differentiated metabolic changes between the Sham and TBI groups. Fourteen amine metabolites (including isomers such as L‐leucine and L‐isoleucine) are significantly altered in specific regions. The metabolic changes are well matched with the degree of neuronal damage, glia activation, and neurorestoration. XFZYD reverses the dysregulation of several amine metabolites, such as hippocampal Lys‐Phe/Phe‐Lys and dopamine. Also, XFZYD enhances post‐TBI angiogenesis in the hippocampus and the thalamus. Conclusion This study reveals the local amine‐metabolite and histological changes in the subacute stage of TBI. XFZYD may promote TBI recovery by normalizing amine metabolites and spatially promoting dopamine production and angiogenesis.
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