Connexin43 reduces LPS‐induced inflammation in hDPCs through TLR4‐NF‐κB pathway via hemichannels

炎症 TLR4型 脂多糖 化学 牙髓(牙) NF-κB 细胞生物学 促炎细胞因子 细胞因子 免疫学 医学 生物 病理
作者
Ping Long,Lin Xiong,Changhai Ding,Yanli Kuang,Yue He,Guangwen Li,Jingang Xiao,Shiting Li
出处
期刊:Oral Diseases [Wiley]
标识
DOI:10.1111/odi.14767
摘要

Abstract Objectives Connexin43 (Cx43) is involved in the inflammation of many tissue types. Dental caries is infectious disease resulting from mineralized tissue dissolution by a specific bacterial population, causing pulp inflammation. However, Cx43's role in dental pulp remains unclear. Here, we investigated the function of Cx43 during pulp inflammation. Materials and Methods We constructed a dentin injury model in Sprague–Dawley rats to investigate changes in Cx43 expression during pulp inflammation. Cx43 was inhibited in human dental pulp cells (hDPCs) that had been stimulated with lipopolysaccharide (LPS) to investigate the effect of Cx43 on inflammatory response. Promotion of TLR4‐NF‐κB pathway activity and special Cx43 channel inhibitors were used to clarify the function of Cx43 in hDPCs. Results Dentin injury led to low‐level inflammation in dental pulp. Following dentin injury, Cx43 expression initially decreased before gradually recovering to normal levels. Cx43 inhibition reduced LPS‐induced expression of inflammatory cytokines and NF‐κB pathway activity. Promotion of NF‐κB pathway activity counteracted the effect of Cx43 in hDPCs. Furthermore, inhibition of Cx43 hemichannels reduced LPS‐induced inflammatory cytokine expression. Conclusions Cx43 is involved in inflammation of dental pulp, while its inhibition reduced LPS‐induced inflammation in hDPCs through NF‐κB pathway via blockage of hemichannels.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
2秒前
张嘟嘟发布了新的文献求助10
3秒前
我爱科研发布了新的文献求助20
3秒前
菠萝吹雪发布了新的文献求助10
4秒前
威武的板凳完成签到,获得积分20
4秒前
星辰大海应助张继国采纳,获得10
4秒前
5秒前
么子完成签到,获得积分10
5秒前
赘婿应助Yingkun_Xu采纳,获得10
7秒前
8秒前
胖达完成签到,获得积分10
8秒前
华仔应助张豪杰采纳,获得10
9秒前
xjcy应助威武的板凳采纳,获得10
10秒前
活力的听露完成签到 ,获得积分10
10秒前
nil完成签到,获得积分20
10秒前
11秒前
Manyiu发布了新的文献求助10
12秒前
郑郑郑幸运完成签到 ,获得积分10
12秒前
nixx驳回了几酌应助
13秒前
皮皮小怪发布了新的文献求助10
14秒前
14秒前
菜大鸭完成签到,获得积分20
14秒前
14秒前
15秒前
喜悦的板凳完成签到 ,获得积分10
16秒前
赘婿应助963采纳,获得10
18秒前
阳光发布了新的文献求助10
18秒前
HUO发布了新的文献求助10
18秒前
33发布了新的文献求助30
19秒前
19秒前
深情的若冰完成签到,获得积分10
20秒前
科研通AI2S应助replay采纳,获得10
21秒前
hhh发布了新的文献求助20
22秒前
吾酒完成签到,获得积分10
23秒前
23秒前
行走的sci完成签到,获得积分10
25秒前
无辜紫菜完成签到,获得积分10
25秒前
小黄同学爱学习完成签到 ,获得积分10
25秒前
26秒前
28秒前
高分求助中
Evolution 10000
ISSN 2159-8274 EISSN 2159-8290 1000
Becoming: An Introduction to Jung's Concept of Individuation 600
Ore genesis in the Zambian Copperbelt with particular reference to the northern sector of the Chambishi basin 500
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
A new species of Velataspis (Hemiptera Coccoidea Diaspididae) from tea in Assam 500
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3162727
求助须知:如何正确求助?哪些是违规求助? 2813601
关于积分的说明 7901404
捐赠科研通 2473189
什么是DOI,文献DOI怎么找? 1316684
科研通“疑难数据库(出版商)”最低求助积分说明 631482
版权声明 602175