S-methyl-L-cysteine sulfoxide as a characteristic marker for rape royal jelly: Insights from untargeted and targeted metabolomic analysis

Among the varieties of royal jelly (RJ), often referred to as "liquid gold", rape royal jelly (RRJ) is popular because of its superior nutritional value. However, existing physicochemical indicators fall short in identifying different types of RJ. Utilizing a UPLC-Q-Exactive Orbitrap-MS technique combined with metabolomics, this study was the first to identify S-methyl-L-cysteine sulfoxide (SMCSO) in RRJ, thereby it from other types of RJ. Subsequent to this observation, a method based on UPLC-QqQ-MS/MS, was developed and optimized for precise SMCSO quantification in RRJ, achieving a detection range of 77.55–112.68 mg/kg. Furthermore, an analysis of honey and bee bread harvested from the same batch of rape plants confirmed the presence of SMCSO, with the highest concentration detected in rape bee bread. In light of these findings, SMCSO emerges as a potent authenticity marker for RRJ.