流式细胞术
同种类的
荧光
人类免疫缺陷病毒(HIV)
材料科学
亚甲蓝
检出限
发光
分子生物学
催化作用
病毒学
化学
生物
色谱法
生物化学
光电子学
物理
量子力学
光催化
热力学
作者
Xiaoqi Ou,Zhengli Wan,Ying Xiong,Ke Huang,Wei Wang,Zulimire Nuermaimaiti,Yanting Chen,Duerdanna Yiliya,Hongyin Lin,Zhixiang Dai,Yi Li,Piaopiao Chen
标识
DOI:10.1021/acsami.3c06742
摘要
Regularly measuring the level of CD4+ cells is necessary for monitoring progression and predicting prognosis in patients suffering from an infection with the human immunodeficiency virus (HIV). However, the current flow cytometry standard detection method is expensive and complicated. A parallel catalytic hairpin assembly (CHA)-assisted fluorescent aptasensor is reported for homogeneous CD4 count by targeting the CD4 protein expressed on the membrane of CD4+ cells. Detection was achieved using CdTe quantum dots (QDs) and methylene blue (MB) as signal reporters. CdTe QDs distinguished CHA-assisted release of Ag+ and C-Ag+-C and MB that has differentiated cytosine (C)-rich single-stranded DNA (ssDNA) and C-Ag+-C, generating changes in fluorescence intensity. With the assistance of the CHA strategy and luminescent nanomaterials, this method reached limits of detection of 0.03 fg/mL for the CD4 protein and 0.3 cells/mL for CD4+ cells with linear ranges of 0.1 to 100 fg/mL and 1 to 1000 cells/mL, respectively. The method was validated in 50 clinical whole blood samples consisting of 30 HIV-positive patients, 10 healthy volunteers, and 10 patients with cancer or other chronic infections. The findings from this method were in good agreement with the data from clinical flow cytometry. Due to its sensitivity, affordability, and ease of operation, the current method has demonstrated great potential for routine CD4 counts for the management of HIV, especially in communities and remote areas.
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