Peripheral Circular RNA Profiling from Patients with Allergic Rhinitis Identified hsa_circRNA_404013 as a Potential Diagnostic Biomarker

环状RNA 生物标志物 小桶 外周血 实时聚合酶链反应 医学 逆转录聚合酶链式反应 基因表达 免疫学 基因 小RNA 生物 基因本体论 遗传学
作者
Jin Peng,Kaiyue Sun,Qinqin Zhang,Chaofan Shen,Yiran Zang,Lili Zhi,Li Shi
出处
期刊:International Archives of Allergy and Immunology [Karger Publishers]
卷期号:183 (10): 1078-1088 被引量:6
标识
DOI:10.1159/000525791
摘要

The study of peripheral circular RNA (circRNA) expression profile in patients with allergic rhinitis (AR) was absent to date, and we aimed to obtain the circRNA expression profile and identify the candidate biomarker from AR.circRNA chip was performed to screen differentially expressed circRNAs in the peripheral blood sample from AR patients and healthy controls. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathways were analyzed to further search the function of the differential expressed circRNAs. The real-time quantitative reverse transcription-polymerase chain reaction was further used to verify the candidate circRNA and also analyze its potential correlation with clinical parameters.Significantly up-regulated expression level of hsa_circRNA_404013 in AR was obtained by circRNA chip and further verified in 79 AR patients and 48 healthy controls. hsa_circRNA_404013 was significantly positively correlated with nasal discharge, nasal itching, the total nasal symptoms of AR, and brain-derived neurotrophic factor (BDNF) expression level in peripheral blood. Receiver operating characteristic curve analysis results showed that hsa_circRNA_404013 may be used as peripheral blood circulating marker for the diagnosis of AR with the area under curve of 0.8499 (95% CI: 0.783-0.916). In further bioinformatics analysis, hsa_circRNA_404013 may regulate the expression of BDNF through hsa-mir-182-5p contributing to the pathogenesis of AR.The expression profile of circRNAs from the peripheral blood sample of AR patients was obtained. The expression of hsa_circRNA_404013 was significantly up-regulated in the peripheral blood of AR patients, which may be used as a circulating marker for AR patients. Furthermore, hsa_circRNA_404013 may regulate the expression level of BDNF through hsa-mir-182-5p in AR pathogenesis.
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