Metagenomic strategies uncover the soil bioavailable phosphorus improved by organic fertilization in Mollisols

软土 矿化(土壤科学) 肥料 微生物种群生物学 基因组 土壤微生物学 生物利用度 营养物 农业土壤学 肥料 自行车 微生物 土壤肥力 相对物种丰度 营养循环 环境化学 生态学 丰度(生态学) 农学 化学 生物 土壤水分 细菌 土壤生物多样性 生物信息学 遗传学 基因 历史 考古 有机化学
作者
Xiaojing Hu,Haidong Gu,Junjie Liu,Dan Wei,Ping Zhu,Xian Cui,Baoku Zhou,Xueli Chen,Jian Jin,Xiaobing Liu,Guanghua Wang
出处
期刊:Agriculture, Ecosystems & Environment [Elsevier BV]
卷期号:349: 108462-108462 被引量:31
标识
DOI:10.1016/j.agee.2023.108462
摘要

Microorganisms play essential roles in soil phosphorus (P) cycling and the regulation of P bioavailability, however, genetic information on microbial P cycling in response to nutrient inputs is largely unclear. Here, metagenomic sequencing and genome binning were used to investigate microbial functional traits under chemical and organic fertilization in three long-term field experiments across black soil region of Northeast China. The results revealed that manure amendments strongly affected microbial P cycle-related functional gene patterns, which were significantly and positively correlated with the contents of soil total P (TP) and available P (AP). Manure addition directly increased soil AP concentrations, and indirectly acted through the alterations of microbial functional genes involved in soil P cycling. Specifically, manure amendments consistently decreased abundances of phnC gene and increased gene abundances of phnP, opd, and phoN across three locations, suggesting the potentially inhibition of soil microbial P-uptake and transport and the promotion of soil microbial organic P- mineralization. Manure addition promoted microbial inorganic P-solubilization by enriching the ppa, gcd, and pqqC genes at two out of three locations, while chemical fertilizer (CF) addition slightly stimulated the functional gene abundances involved in microbial P-uptake and transport and P-starvation response regulation. In addition, soil AP content was negatively correlated with the phnC gene abundance but positively correlated with the gene abundances of opd and phoN. Moreover, 23 metagenome-assembled genomes (MAGs) were reconstructed covering all soil samples, all of which contained the phnC gene with the copy numbers varying from 1 to 19. Nevertheless, only bin44 had a negative correlation with soil AP (r = −0.361, P = 0.030) and could be considered as a potential indicator regulating microbial P-uptake and transport. Taken together, manure inputs positively accelerated microbial P-transformations, which was beneficial for the establishment of efficient P management strategies in sustainable-intensive agriculture.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
liyan完成签到 ,获得积分10
1秒前
2秒前
嗯啊完成签到,获得积分10
4秒前
酷波er应助immm采纳,获得10
5秒前
优雅含莲完成签到 ,获得积分10
5秒前
呜啦啦完成签到,获得积分10
6秒前
6秒前
lulu8809完成签到,获得积分10
9秒前
9秒前
二十五完成签到,获得积分10
10秒前
romeo完成签到,获得积分10
11秒前
kaka完成签到 ,获得积分10
11秒前
Akim应助xialuoke采纳,获得10
11秒前
昏睡的蟠桃应助guoxingliu采纳,获得200
12秒前
慕容松完成签到,获得积分10
13秒前
romeo发布了新的文献求助10
13秒前
ss_hHe完成签到,获得积分10
14秒前
14秒前
15秒前
zjcomposite完成签到,获得积分10
15秒前
nn发布了新的文献求助10
15秒前
css完成签到,获得积分10
15秒前
大橙子发布了新的文献求助10
16秒前
1111完成签到,获得积分10
16秒前
敏er好学完成签到,获得积分10
17秒前
细腻的谷秋完成签到 ,获得积分10
17秒前
独特的易形完成签到,获得积分10
18秒前
yangyangyang完成签到,获得积分0
21秒前
yirenli完成签到,获得积分10
22秒前
叶子完成签到 ,获得积分10
22秒前
angel完成签到,获得积分10
24秒前
正经大善人完成签到,获得积分10
26秒前
动听的秋白完成签到 ,获得积分10
27秒前
汉堡包应助biofresh采纳,获得30
27秒前
自然归尘完成签到 ,获得积分10
28秒前
缓慢海蓝完成签到 ,获得积分10
30秒前
liyiren完成签到,获得积分10
31秒前
31秒前
zhaopeipei完成签到,获得积分10
31秒前
量子星尘发布了新的文献求助10
32秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Handbook of Industrial Diamonds.Vol2 1100
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038201
求助须知:如何正确求助?哪些是违规求助? 3575940
关于积分的说明 11373987
捐赠科研通 3305747
什么是DOI,文献DOI怎么找? 1819274
邀请新用户注册赠送积分活动 892662
科研通“疑难数据库(出版商)”最低求助积分说明 815022