生物
类胡萝卜素
拟南芥
八氢番茄红素合酶
生物合成
叶绿体
拟南芥
染色体体
植物烯
生物化学
质体
酶
基因
突变体
作者
Sombir Rao,Hongbo Cao,Franz Joseph O’Hanna,Xuesong Zhou,Andy C. W. Lui,Emalee Wrightstone,Tara Fish,Yong Yang,Theodore W. Thannhauser,Lailiang Cheng,Natalia Dudareva,Li Li
出处
期刊:The Plant Cell
[Oxford University Press]
日期:2024-01-31
卷期号:36 (5): 1868-1891
被引量:6
标识
DOI:10.1093/plcell/koae030
摘要
Abstract Carotenoids are essential for photosynthesis and photoprotection. Plants must evolve multifaceted regulatory mechanisms to control carotenoid biosynthesis. However, the regulatory mechanisms and the regulators conserved among plant species remain elusive. Phytoene synthase (PSY) catalyzes the highly regulated step of carotenogenesis and geranylgeranyl diphosphate synthase (GGPPS) acts as a hub to interact with GGPP-utilizing enzymes for the synthesis of specific downstream isoprenoids. Here, we report a function of Nudix hydrolase 23 (NUDX23), a Nudix domain-containing protein, in post-translational regulation of PSY and GGPPS for carotenoid biosynthesis. NUDX23 expresses highly in Arabidopsis (Arabidopsis thaliana) leaves. Overexpression of NUDX23 significantly increases PSY and GGPPS protein levels and carotenoid production, whereas knockout of NUDX23 dramatically reduces their abundances and carotenoid accumulation in Arabidopsis. NUDX23 regulates carotenoid biosynthesis via direct interactions with PSY and GGPPS in chloroplasts, which enhances PSY and GGPPS protein stability in a large PSY–GGPPS enzyme complex. NUDX23 was found to co-migrate with PSY and GGPPS proteins and to be required for the enzyme complex assembly. Our findings uncover a regulatory mechanism underlying carotenoid biosynthesis in plants and offer promising genetic tools for developing carotenoid-enriched food crops.
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