The Transcription Factor WFZP Interacts with the Chromatin Remodeler TaSYD to Regulate Root Architecture and Nitrogen Uptake Efficiency in Wheat

The root architecture is crucial for the robust growth and nutrient absorption in cereals. However, it is urgent to identify the factors that simultaneously optimize root architecture and nutrient utilization in wheat. In this study, a beneficial role of the class II AP2/ERF transcription factor WHEAT FRIZZY PANICLE (WFZP) on lateral root number (LRN), root length (RL), and nitrogen utilization is revealed. In addition, interactors of WFZP including TaSYD are identified, as a subunit of the chromatin remodeling complex. The Tasyd mutants show a significant reduction in LRN, RL, and nitrogen uptake efficiency, resembling the phenotype of wfzp mutants. Furthermore, it is revealed that the WFZP-TaSYD module promotes the expression of root development and nitrate uptake-related genes by modulating chromatin accessibility and histone modifications. Finally, an elite allele (WFZP-A-I) associated with improved LRN and thousand-grain weight (TGW) is identified. Hence, these findings not only unveil the mechanisms underlying the coordination of root development and nitrogen uptake efficiency, but also provide valuable targets for breeding high-yield crops.