Tissue clearing and three-dimensional imaging of intact tissues: a review on FACT protocol

清理 协议(科学) 病理 医学 化学 财务 经济 替代医学
作者
Zohreh Farrar,Alireza Afshar,Afshin Zare,Nadiar M. Mussin,Asset A. Kaliyev,Kulyash R. Zhilisbayeva,Mahdi Mahdipour,Amin Tamadon
出处
期刊:Journal of Histotechnology [Taylor & Francis]
卷期号:47 (3): 126-142
标识
DOI:10.1080/01478885.2024.2352695
摘要

FACT is a developed technique for clearing tissues that does not use acrylamide. Since the removal of lipids is crucial for transparency and efficient antibody staining throughout the tissue, especially for microscopy and imaging, it is a harmful process that can cause the loss of important biological molecules such as proteins. The FACT technique overcomes this by chemically bonding the membrane and intracellular proteins with the extracellular matrix, creating a massive 3D hydrogel matrix and providing structural support to fortify the tissue during processing. Compared to other acrylamide-based techniques, the FACT technique requires less labor and harmful chemicals and is therefore considered a more suitable option. In this study, we describe the complete FACT protocol for antibody staining and imaging of whole-cleared tissues while preserving structure and improving image quality. The protocol includes tissue perfusion, fixation, clearing, antibody staining, refractive index matching (RIM) (), microscopy, and imaging. The timing for each step varies depending on the size, weight, and type of tissue, as well as the type of immunostaining. We provide an example of the FACT protocol using mouse brain tissue, which demonstrates its suitability for molecular interrogation analysis of large tissues. The FACT technique has been successfully performed on different types of tissues, making it a favorable choice for a variety of applications.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
上官若男应助曾经晓亦采纳,获得10
3秒前
Jasper应助翁雁丝采纳,获得10
3秒前
菠萝菠萝发布了新的文献求助10
4秒前
5秒前
简单发布了新的文献求助10
5秒前
DD完成签到,获得积分20
6秒前
爱自己就好完成签到,获得积分10
7秒前
可靠的雪碧完成签到,获得积分10
7秒前
西瓜汽水发布了新的文献求助10
9秒前
11秒前
无花果应助稀罕你采纳,获得10
11秒前
11秒前
11秒前
12秒前
13秒前
14秒前
nnnn发布了新的文献求助30
15秒前
15秒前
林机一动发布了新的文献求助10
17秒前
niki发布了新的文献求助10
17秒前
隐形曼青应助翁雁丝采纳,获得10
17秒前
18秒前
sbw发布了新的文献求助10
19秒前
852应助北彧采纳,获得10
20秒前
Ava应助水天采纳,获得10
20秒前
paulmichael发布了新的文献求助10
21秒前
21秒前
21秒前
bird完成签到,获得积分10
22秒前
23秒前
zfm发布了新的文献求助10
24秒前
24秒前
26秒前
结实伯云完成签到,获得积分10
27秒前
17发布了新的文献求助10
27秒前
胡乱说兔的熊完成签到,获得积分10
28秒前
翁雁丝发布了新的文献求助10
29秒前
30秒前
结实伯云发布了新的文献求助10
31秒前
量子星尘发布了新的文献求助10
32秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 700
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3975722
求助须知:如何正确求助?哪些是违规求助? 3520056
关于积分的说明 11200719
捐赠科研通 3256455
什么是DOI,文献DOI怎么找? 1798271
邀请新用户注册赠送积分活动 877490
科研通“疑难数据库(出版商)”最低求助积分说明 806390