亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Tissue clearing and three-dimensional imaging of intact tissues: a review on FACT protocol

清理 协议(科学) 病理 医学 化学 财务 经济 替代医学
作者
Zohreh Farrar,Alireza Afshar,Afshin Zare,Nadiar M. Mussin,Asset A. Kaliyev,Kulyash R. Zhilisbayeva,Mahdi Mahdipour,Amin Tamadon
出处
期刊:Journal of Histotechnology [Taylor & Francis]
卷期号:47 (3): 126-142
标识
DOI:10.1080/01478885.2024.2352695
摘要

FACT is a developed technique for clearing tissues that does not use acrylamide. Since the removal of lipids is crucial for transparency and efficient antibody staining throughout the tissue, especially for microscopy and imaging, it is a harmful process that can cause the loss of important biological molecules such as proteins. The FACT technique overcomes this by chemically bonding the membrane and intracellular proteins with the extracellular matrix, creating a massive 3D hydrogel matrix and providing structural support to fortify the tissue during processing. Compared to other acrylamide-based techniques, the FACT technique requires less labor and harmful chemicals and is therefore considered a more suitable option. In this study, we describe the complete FACT protocol for antibody staining and imaging of whole-cleared tissues while preserving structure and improving image quality. The protocol includes tissue perfusion, fixation, clearing, antibody staining, refractive index matching (RIM) (), microscopy, and imaging. The timing for each step varies depending on the size, weight, and type of tissue, as well as the type of immunostaining. We provide an example of the FACT protocol using mouse brain tissue, which demonstrates its suitability for molecular interrogation analysis of large tissues. The FACT technique has been successfully performed on different types of tissues, making it a favorable choice for a variety of applications.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
量子星尘发布了新的文献求助10
3秒前
3秒前
7秒前
17秒前
lijiawei发布了新的文献求助10
19秒前
Criminology34发布了新的文献求助50
21秒前
睡觉补充能量完成签到,获得积分10
21秒前
fengfenghao完成签到,获得积分10
27秒前
小凯完成签到 ,获得积分10
30秒前
jueshadi完成签到 ,获得积分10
30秒前
朵朵完成签到,获得积分10
32秒前
37秒前
41秒前
dong发布了新的文献求助10
42秒前
44秒前
44秒前
44秒前
aaaaal发布了新的文献求助10
46秒前
48秒前
annaanna完成签到 ,获得积分10
50秒前
Criminology34发布了新的文献求助50
52秒前
ccc完成签到 ,获得积分10
52秒前
54秒前
YY发布了新的文献求助10
54秒前
Gin发布了新的文献求助10
57秒前
aaaaal完成签到,获得积分10
57秒前
Becky完成签到 ,获得积分10
1分钟前
1分钟前
杨北风完成签到 ,获得积分20
1分钟前
科研通AI6应助科研通管家采纳,获得10
1分钟前
1分钟前
共享精神应助科研通管家采纳,获得10
1分钟前
1分钟前
所所应助科研通管家采纳,获得10
1分钟前
1分钟前
1分钟前
Hello应助科研通管家采纳,获得10
1分钟前
小蘑菇应助科研通管家采纳,获得10
1分钟前
1分钟前
ye发布了新的文献求助20
1分钟前
高分求助中
(应助此贴封号)【重要!!请各用户(尤其是新用户)详细阅读】【科研通的精品贴汇总】 10000
Architectural Corrosion and Critical Infrastructure 1000
Early Devonian echinoderms from Victoria (Rhombifera, Blastoidea and Ophiocistioidea) 1000
By R. Scott Kretchmar - Practical Philosophy of Sport and Physical Activity - 2nd (second) Edition: 2nd (second) Edition 666
Electrochemistry: Volume 17 600
Physical Chemistry: How Chemistry Works 500
SOLUTIONS Adhesive restoration techniques restorative and integrated surgical procedures 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 内科学 生物化学 物理 计算机科学 纳米技术 遗传学 基因 复合材料 化学工程 物理化学 病理 催化作用 免疫学 量子力学
热门帖子
关注 科研通微信公众号,转发送积分 4944655
求助须知:如何正确求助?哪些是违规求助? 4209521
关于积分的说明 13085355
捐赠科研通 3989302
什么是DOI,文献DOI怎么找? 2184055
邀请新用户注册赠送积分活动 1199418
关于科研通互助平台的介绍 1112457